Introduction to Aspen Chromatography

Course Id:  EAP289   |   Duration:  2.00 day(s)   |   CEUs Awarded:  1.4   |   Level:  Intermediate

Course Objective

Learn how to set up and solve the dynamic simulation problems in chromatography. Gain first-hand experience in working within the Aspen Chromatography simulation environment. Apply Aspen Chromatography to build and execute simulations rapidly.

Course Overview

  • To provide customers with all the skills and understanding required to set up and solve their own dynamic simulation problems using Aspen Chromatography
  • Develop familiarity with the Aspen Chromatography application in  order to access and navigate within the graphical user interface
  • Identify and explain:
  • Modeling conventions used
  • Underlying modeling assumptions
  • Demonstrate how to use the Chromatography model library for:
  • Batch Chromatographic processes
  • Ion Exchange Chromatography
  • Simulated Moving Bed processes (SMB)
  • Parameter estimation
  • Steady state optimization
  • Apply acquired knowledge through hands-on workshops


  • Gain first-hand experience and confidence in working within the Aspen Chromatography simulation environment
  • Apply Aspen Chromatography to build and execute simulations rapidly
  • Enhance the customer’s ability to succeed in their application of Aspen Chromatography, and thus reap the technical and business benefits as soon as possible


Scientists and engineers involved in the design, operation, optimization, and analysis of industrial chromatographic processes such as batch or gradient elution chromatography and simulated moving bed.


  • Instruction on basic topics
  • Discussion about the general approach and the key elements for successful simulations
  • Demonstrations of features
  • Instructor-guided exercises
  • Hands-on workshops that apply learned concepts
  • Detailed course notes


  • No prior knowledge of dynamic simulation required
  • No prior experience of Aspen Chromatography is required
  • Some familiarity with basic Microsoft® Windows skills are helpful
  • Understanding of industrial adsorption/ chromatography processes are helpful
  • EAP2311 Building Custom Simulation Models using Aspen Custom Modeler

Class Schedule

Class Agenda

EAP289: Introduction to Aspen Chromatography

Graphical User Interface Basics and Physical Properties

  • Learn to access and navigate the Aspen Chromatography graphical user interface
  • Build flowsheets to simulate typical processes
  • Create plots and tables to analyze simulation results
  • Access runtime and solver options to ensure simulation convergence  

Batch Column Basics
  • Discuss and review the batch column capabilities
  • Define the column assumptions required to establish a simulation
  • Show how to create a flowsheet using the batch column and other supporting models
  • Review results and chromatography report
  • Complete the workshop to gain familiarity with the batch column simulation setup and analysis of a chromatographic separation of D- & L- threonine
  • Workshop: Chromatographic Separation of D- & L- Threonine
  • Workshop(Optional) Batch Injection of Sugars

Cyclic Operation
  • Demonstrate how to use the Cycle Organizer to create cyclic processes
  • Gain familiarity with simulating successive batch injection
  • Gain familiarity with simulating successive batch injection with product collection

Simulated Moving Bed (SMB) and True Moving Bed (TMB)
  • Examine the TMB/SMB capabilities of Aspen Chromatography
  • Set up and configure TMB/SMB flowsheets
  • Demonstrate how to use the SMB flowrate optimizer
  • Recognize what results are available and how to interpret them
  • Discuss how to use the results predicted from TMB operation to initialize SMB operation to achieve faster convergence to cyclic steady-state
  • Complete workshop to gain familiarity with separation of D- and L- threonine enantiomers using a continuous counter-current separator
  • Workshop: Separation of D- and L- Threonine Enantiomers Using A Continuous
  • Counter-Current Separator

User Correlations Through Flowsheet Constraints
  • Identify and explain flowsheet constraints
  • Determine when flowsheet constraints can be used
  • Identify and explain custom isotherms
  • Demonstrate how to write custom isotherms using the modeling language
  • Complete the workshop to create the extended Langmuir isotherm as a flowsheet constraint
  • Workshop: Create the Extended Langmuir Isotherm as a Flowsheet Constraint

Size Exclusion Chromatography
  • Discuss and review Size Exclusion Chromatography (SEC):
  • Advantages and disadvantages of SEC
  • Factors that affect the performance of SEC
  • Aspen Chromatography assumptions for modeling SEC
  • Demonstration to create and execute a SEC simulation (Batch SEC of Myoglobin and Ovalbumin)

Affinity Chromatography
  • Discuss and review Affinity Chromatography:
  • Advantages and disadvantages of Affinity Chromatography
  • Operational steps of Affinity Chromatography
  • How Aspen Chromatography can be used to model Affinity Chromatography systems
  • How to enable and write reactions for the column model
  • Demo to create and execute an Affinity Chromatography simulation (Affinity Chromatography by User Reactions)

Bulk Liquid Operation
  • Examine the difference between the trace and bulk liquid component material balances
  • Discuss implications when using the bulk liquid mode:
  • Demonstration to gain familiarity with Xylene separation using True Moving Bed (TMB)

Ion Exchange Operation
  • Recognize the configuration and specification of ion exchange models
  • Identify the available isotherms
  • Create batch and gradient elution ion-exchange flowsheets
  • Define gradient elution strategies using Cycle Organizer
  • Complete the workshop to gain familiarity with linear gradient elution of the proteins ovalbumin and lactoglobulin
  • Workshop: Linear Gradient Elution of the Proteins Ovalbumin and Lactoglobulin

Parameter Estimation
  • Discuss and review parameter estimation
  • Review the initial guess strategy as it relates to parameter estimation
  • Estimate isotherm parameters using steady-state estimation
  • Demo to gain familiarity with fitting isotherm parameters to static measurements
  • Estimate isotherm parameters using dynamic estimation
  • Complete the workshop to gain familiarity with fitting isotherm parameters  to dynamic breakthrough measurements
  • Workshop: The Fitting of Isotherm Parameters  to Dynamic Breakthrough Measurements

Diagnostic and Solution Methods
  • Discuss and review issues in convergence
  • Provide general advice on troubleshooting
  • Diagnostic tools
  • Solver settings

Aspen Technology, Inc. awards Continuing Education Units (CEUs) for training classes conducted by our organization. One CEU is granted for every 10 hours of class participation.